Role of NF-kB, p53, and p21 in the regulation of TNF-α mediated apoptosis of lymphocytes

N V Ryazantseva; V V Novitskii; O B Zhukova; A K Biktasova; O E Chechina; E V Sazonova; T T Radzivil; A N Wice; N Yu Chasovskikh

doi:10.1007/s10517-010-0873-8

Role of NF-kB, p53, and p21 in the regulation of TNF-α mediated apoptosis of lymphocytes

Bull Exp Biol Med. 2010 Jul;149(1):50-3. doi: 10.1007/s10517-010-0873-8.

[Article in English, Russian]

Authors

N V Ryazantseva¹, V V Novitskii, O B Zhukova, A K Biktasova, O E Chechina, E V Sazonova, T T Radzivil, A N Wice, N Yu Chasovskikh

Affiliation

¹ Siberian State Medical University, Russian Ministry of Health, Tomsk, Russia. mv@mail.ssmu.ru

PMID: 21113457
DOI: 10.1007/s10517-010-0873-8

Abstract

The effect of recombinant TNF-α on programmed death of donor lymphocytes was studied in vitro. The proapoptotic effect of this cytokine is realized through transcription factors and cell cycle inhibitors. Incubation of lymphocytes with recombinant TNF-α revealed increased levels of NF-kB and p21 and reducted content of nonphosphorylated p53.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Apoptosis / drug effects*
Apoptosis / physiology
Cyclin-Dependent Kinase Inhibitor p21 / metabolism*
Female
Flow Cytometry
Humans
In Vitro Techniques
Lymphocytes / drug effects*
Lymphocytes / physiology
Male
NF-kappa B / metabolism*
Recombinant Proteins / pharmacology*
Statistics, Nonparametric
Tumor Necrosis Factor-alpha / pharmacology*
Tumor Necrosis Factor-alpha / physiology
Tumor Suppressor Protein p53 / metabolism*

Substances

Cyclin-Dependent Kinase Inhibitor p21
NF-kappa B
Recombinant Proteins
Tumor Necrosis Factor-alpha
Tumor Suppressor Protein p53