A sub-nanomole level electrochemical method for determination of prochloraz and its metabolites based on medical stone doped disposable electrode

Talanta. 2010 Dec 15;83(2):591-5. doi: 10.1016/j.talanta.2010.10.002. Epub 2010 Oct 8.

Abstract

A ultrasensitive, simple and convenient electrochemical method was firstly developed for the determination of prochloraz and its metabolites as 2,4,6-trichlorophenol (2,4,6-TCP) using nano-aperture medical stone. Compared with the undoped disposable electrode (UDE), nano-aperture medical stone doped disposable electrode (MSDDE) not only significantly enhances the oxidation peak current of 2,4,6-TCP but also lowers the oxidation overpotential, suggesting that the nano-aperture MSDDE can remarkably improve the sensitivity of 2,4,6-TCP. The experimental conditions such as pH values of buffer solution, the content of nano-aperture medical stone, accumulation potential and time were optimized for the determination of 2,4,6-TCP. At optimal conditions, the oxidation peak current is proportional to the concentration of 2,4,6-TCP over the range from 6.0 × 10(-9) to 8.0 × 10(-5)mol L(-1). Finally, this novel method was successfully employed to detect prochloraz and its metabolites in orange rind with the detection limit of 8.4 × 10(-10)mol L(-1) (0.3 ng g(-1)) and the method was validated by gas chromatography.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calibration
  • Chemistry Techniques, Analytical
  • Chlorophenols / chemistry
  • Chromatography, Gas / methods
  • Citrus sinensis / metabolism
  • Electrochemistry / methods*
  • Electrodes
  • Hydrogen-Ion Concentration
  • Imidazoles / analysis*
  • Imidazoles / chemistry
  • Microscopy, Electron, Transmission / methods

Substances

  • Chlorophenols
  • Imidazoles
  • prochloraz
  • 2,4,6-trichlorophenol