Amplified electrochemical DNA sensor using peroxidase-like DNAzyme

Talanta. 2010 Dec 15;83(2):500-4. doi: 10.1016/j.talanta.2010.09.054. Epub 2010 Oct 23.

Abstract

A novel electrochemical DNA sensor was developed here by using peroxidase-like G-quadruplex-based DNAzyme as a biocatalytic label. A hairpin structure including the G-quadruplex-based DNAzyme in a caged configuration and the target DNA probe were immobilized on Au-electrode surface. Upon hybridization with the target, the hairpin structure was opened, and the G-quadruplex-based DNAzyme was generated on the electrode surface, triggering the electrochemical oxidization of hydroquinone by H(2)O(2), which provide a quantitative measure for the detection of the target DNA. The DNA target was analyzed with a detection limit of 0.6 nM. This method is simple and easy to design without direct conjugation of redox-active element.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / methods*
  • Catalysis
  • DNA / chemistry*
  • DNA / genetics
  • DNA, Catalytic / chemistry*
  • Electrochemistry / methods*
  • G-Quadruplexes
  • Hydrogen Peroxide / chemistry
  • Hydroquinones / chemistry
  • Models, Genetic
  • Nucleic Acid Hybridization
  • Nucleic Acids / chemistry
  • Oligonucleotides / genetics
  • Time Factors

Substances

  • DNA, Catalytic
  • Hydroquinones
  • Nucleic Acids
  • Oligonucleotides
  • DNA
  • Hydrogen Peroxide
  • hydroquinone