Chemoattractant-mediated Rap1 activation requires GPCR/G proteins

Mol Cells. 2010 Dec;30(6):563-7. doi: 10.1007/s10059-010-0153-5. Epub 2010 Nov 18.

Abstract

Rap1 is rapidly activated upon chemoattractant stimulation and plays an important role in cell adhesion and cytoskeletal reorganization during chemotaxis. Here, we demonstrate that G-protein coupled receptors and G-proteins are essential for chemoattractant-mediated Rap1 activation in Dictyostelium. The rapid Rap1 activation upon cAMP chemoattractant stimulation was absent in cells lacking chemoattractant cAMP receptors cAR1/cAR3 or a subunit of the heterotrimeric G-protein complex Gα2. Loss of guanylyl cyclases GCA/SGC or a cGMP-binding protein GbpC exhibited no effect on Rap1 activation kinetics. These results suggest that Rap1, a key regulator for the regulation of cytoskeletal reorganization during cell movement, is activated through the G-protein coupled receptors cAR1/cAR3 and Gα2 proteins in a way independent of the cGMP signaling pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium-Binding Proteins / genetics
  • Calcium-Binding Proteins / metabolism
  • Carrier Proteins / metabolism
  • Cell Adhesion
  • Cell Movement
  • Chemotactic Factors / metabolism*
  • Cyclic AMP / metabolism
  • Dictyostelium / cytology
  • Dictyostelium / genetics
  • Dictyostelium / metabolism*
  • GTP-Binding Protein alpha Subunits / genetics
  • GTP-Binding Protein alpha Subunits / metabolism
  • GTP-Binding Proteins / metabolism*
  • Guanylate Cyclase / genetics
  • Guanylate Cyclase / metabolism
  • Intracellular Signaling Peptides and Proteins / metabolism
  • Kinetics
  • Receptors, Cyclic AMP / genetics
  • Receptors, Cyclic AMP / metabolism*
  • Receptors, G-Protein-Coupled / metabolism*
  • Shelterin Complex
  • Signal Transduction
  • Telomere-Binding Proteins / genetics
  • Telomere-Binding Proteins / metabolism*

Substances

  • Calcium-Binding Proteins
  • Carrier Proteins
  • Chemotactic Factors
  • GCA protein, human
  • GTP-Binding Protein alpha Subunits
  • Intracellular Signaling Peptides and Proteins
  • Receptors, Cyclic AMP
  • Receptors, G-Protein-Coupled
  • Shelterin Complex
  • TERF2IP protein, human
  • Telomere-Binding Proteins
  • cyclic AMP receptor cAR1
  • cyclic GMP-binding protein
  • Cyclic AMP
  • GTP-Binding Proteins
  • SgcA protein, Dictyostelium discoideum
  • Guanylate Cyclase