Abstract
Rap1 is rapidly activated upon chemoattractant stimulation and plays an important role in cell adhesion and cytoskeletal reorganization during chemotaxis. Here, we demonstrate that G-protein coupled receptors and G-proteins are essential for chemoattractant-mediated Rap1 activation in Dictyostelium. The rapid Rap1 activation upon cAMP chemoattractant stimulation was absent in cells lacking chemoattractant cAMP receptors cAR1/cAR3 or a subunit of the heterotrimeric G-protein complex Gα2. Loss of guanylyl cyclases GCA/SGC or a cGMP-binding protein GbpC exhibited no effect on Rap1 activation kinetics. These results suggest that Rap1, a key regulator for the regulation of cytoskeletal reorganization during cell movement, is activated through the G-protein coupled receptors cAR1/cAR3 and Gα2 proteins in a way independent of the cGMP signaling pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Calcium-Binding Proteins / genetics
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Calcium-Binding Proteins / metabolism
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Carrier Proteins / metabolism
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Cell Adhesion
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Cell Movement
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Chemotactic Factors / metabolism*
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Cyclic AMP / metabolism
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Dictyostelium / cytology
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Dictyostelium / genetics
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Dictyostelium / metabolism*
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GTP-Binding Protein alpha Subunits / genetics
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GTP-Binding Protein alpha Subunits / metabolism
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GTP-Binding Proteins / metabolism*
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Guanylate Cyclase / genetics
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Guanylate Cyclase / metabolism
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Intracellular Signaling Peptides and Proteins / metabolism
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Kinetics
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Receptors, Cyclic AMP / genetics
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Receptors, Cyclic AMP / metabolism*
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Receptors, G-Protein-Coupled / metabolism*
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Shelterin Complex
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Signal Transduction
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Telomere-Binding Proteins / genetics
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Telomere-Binding Proteins / metabolism*
Substances
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Calcium-Binding Proteins
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Carrier Proteins
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Chemotactic Factors
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GCA protein, human
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GTP-Binding Protein alpha Subunits
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Intracellular Signaling Peptides and Proteins
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Receptors, Cyclic AMP
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Receptors, G-Protein-Coupled
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Shelterin Complex
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TERF2IP protein, human
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Telomere-Binding Proteins
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cyclic AMP receptor cAR1
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cyclic GMP-binding protein
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Cyclic AMP
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GTP-Binding Proteins
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SgcA protein, Dictyostelium discoideum
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Guanylate Cyclase