Diagnosis of foot-and-mouth disease by electrochemical enzyme-linked immunoassay

Annu Int Conf IEEE Eng Med Biol Soc. 2010:2010:674-6. doi: 10.1109/IEMBS.2010.5626230.

Abstract

The development of an inmunosensor for the point-of-care detection of the foot-and-mouth cattle disease is presented. The detector is based on an ELISA method with electrochemical detection. A non-structural protein, 3ABC, is used to selectively detect antibodies is used to selectively detect anti-3ABC antibodies produced after infection. The biological test is performed onto a screen printed electrodes. A dedicated small, portable potentiostat is employed for the control of the sensors, as well as data acquisition, processing, and storage.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / analysis
  • Antibodies / immunology*
  • Conductometry / instrumentation*
  • Enzyme-Linked Immunosorbent Assay / instrumentation*
  • Equipment Design
  • Equipment Failure Analysis
  • Foot-and-Mouth Disease / diagnosis*
  • Foot-and-Mouth Disease / immunology*
  • Humans
  • Point-of-Care Systems*
  • Viral Nonstructural Proteins / blood
  • Viral Nonstructural Proteins / immunology*

Substances

  • 3ABC protein, virus
  • Antibodies
  • Viral Nonstructural Proteins