Abstract
We report the first evidence of a mitochondrial NO synthase (mtNOS) in bird skeletal muscle. In vitro, mtNOS activity stimulated by L-arginine reduced intermyofibrillar mitochondrial oxygen uptake and ATP synthesis rates, stimulated endogenous H(2)O(2) generation, but had no effect on oxidative phosphorylation efficiency. Arginine-induced effects were fully reversed by L-NAME, a known NOS inhibitor. When ducklings were cold exposed for 4 weeks, muscle mitochondria displayed an increased state 3 respiration, a reduced H(2)O(2) generation but no significant alteration in mtNOS activity. We conclude that mtNOS is expressed in avian skeletal muscle.
Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acclimatization
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Animals
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Animals, Newborn
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Anseriformes / metabolism*
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Arginine / pharmacology
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Avian Proteins / antagonists & inhibitors
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Avian Proteins / metabolism*
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Cold Temperature
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Enzyme Inhibitors / pharmacology
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Female
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Hydrogen Peroxide / metabolism
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Male
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Mitochondria, Muscle / drug effects
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Mitochondria, Muscle / enzymology*
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Mitochondria, Muscle / metabolism
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Muscle, Skeletal / enzymology
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NG-Nitroarginine Methyl Ester / pharmacology
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Nitric Oxide Synthase / antagonists & inhibitors
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Nitric Oxide Synthase / metabolism*
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Oxidative Phosphorylation / drug effects
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Oxygen Consumption / drug effects
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Succinic Acid / metabolism
Substances
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Avian Proteins
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Enzyme Inhibitors
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Arginine
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Succinic Acid
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Hydrogen Peroxide
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Nitric Oxide Synthase
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NG-Nitroarginine Methyl Ester