Abstract
14-3-3 Proteins regulate many cellular processes by binding to phosphorylated proteins. Previous findings suggest a connection between three 14-3-3 isoforms and plant nutrient signaling. To better understand how these 14-3-3s regulate metabolism in response to changes in plant nutrient status, putative new targets involved in nitrogen (N) and sulfur (S) metabolisms have been identified. The interactions between these 14-3-3s and multiple proteins involved in N and S metabolism and altered activity of the target proteins were confirmed in planta. Using a combination of methods, this work elucidates how 14-3-3s function as modulators of plant N and S metabolic pathways.
Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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14-3-3 Proteins / genetics
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14-3-3 Proteins / metabolism*
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Amino Acid Sequence
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Arabidopsis / genetics
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Arabidopsis / growth & development
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Arabidopsis / metabolism*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Blotting, Western
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Gene Expression Regulation, Developmental
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Gene Expression Regulation, Plant
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Hydroponics
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Immunoprecipitation
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Metabolic Networks and Pathways*
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Molecular Sequence Data
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Mutation
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Nitrogen / metabolism
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Phosphates / metabolism
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Plants, Genetically Modified
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Potassium / metabolism
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Protein Binding
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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RNA Interference
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Reverse Transcriptase Polymerase Chain Reaction
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Sulfur / metabolism
Substances
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14-3-3 Proteins
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14-3-3chi protein, Arabidopsis
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14-3-3kappa protein, Arabidopsis
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Arabidopsis Proteins
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Phosphates
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Protein Isoforms
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Sulfur
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Nitrogen
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Potassium