Dynamic self-assembly of nonvolatile solutes via controlled solvent evaporation has been exploited as a simple route to create a variety of hierarchically assembled structures. In this work, two glass slides were used to form a confined space in which a solution of a rodlike nanoparticle, tobacco mosaic virus (TMV), was evaporated to create large-scale stripe patterns. The height and width of the stripes are dependent on the TMV concentration. The large-scale-patterned surfaces can be applied to control surface hydrophobicity and direct the growth of bone marrow stromal cells. We systematically studied the effects of stripe width and height on surface hydrophobicity using optical microscopy, atomic force microscopy, and contact angle measurements. This technique offers a facile approach to form 2D patterns on a large surface from a wide range of proteins as well as other biomacromolecules.