Multiplexed typing of Mycobacterium avium subsp. paratuberculosis types I, II, and III by Luminex xMAP suspension array

Michele Gastaldelli; Elisabetta Stefani; Antonia Anna Lettini; Nicola Pozzato

doi:10.1128/JCM.01761-10

Multiplexed typing of Mycobacterium avium subsp. paratuberculosis types I, II, and III by Luminex xMAP suspension array

J Clin Microbiol. 2011 Jan;49(1):389-91. doi: 10.1128/JCM.01761-10. Epub 2010 Nov 17.

Authors

Michele Gastaldelli¹, Elisabetta Stefani, Antonia Anna Lettini, Nicola Pozzato

Affiliation

¹ Istituto Zooprofilattico Sperimentale delle Venezie-Sezione di Verona, Via San Giacomo 5, 37135 Verona, Italy.

Abstract

Differentiation among types I, II, and III is the primary step in typing Mycobacterium avium subsp. paratuberculosis. We propose an innovative approach based on detection of gyrase B (gyrB) gene polymorphisms by suspension array technology, with high discriminatory power and high-throughput potential.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Proteins / genetics
Bacterial Typing Techniques / methods*
DNA Gyrase / genetics
Mycobacterium avium subsp. paratuberculosis / classification*
Mycobacterium avium subsp. paratuberculosis / genetics
Polymorphism, Genetic*
Sensitivity and Specificity

Substances

Bacterial Proteins
DNA Gyrase