The European Water Framework Directive (WFD) establishes a framework for the protection of water bodies. The aim of the Water Framework Directive (WFD) is to ensure sustainable management of groundwater, freshwater and marine water in the European Union, such that a minimum "good ecological status" will be obtained by 2015. We used background morphometric, hydrologic, physico-chemical and biological data, in order to highlight the ecological status of the natural Greek lakes using also the Driving-Pressure-State-Impact-Response (DPSIR) approach in order to link the driving forces and further the pressures with the present state of the lakes' ecosystems. In addition detailed biodiversity and habitat typology data are provided. The assessment of the DPSIR approach under the view of WFD provided a quite clear identification of pressures and the subsequent impacts of the qualitative status of the Greek lakes.