Development of a modified DNA extraction method for pulsed-field gel electrophoresis analysis of Staphylococcus aureus and enterococci without using lysostaphin

Mohammad Mehdi Feizabadi; Arash Ghodousi; Bizhan Nomanpour; Maryam Omrani; Fereshteh Shahcheraghi

doi:10.1016/j.mimet.2010.11.001

Development of a modified DNA extraction method for pulsed-field gel electrophoresis analysis of Staphylococcus aureus and enterococci without using lysostaphin

J Microbiol Methods. 2011 Jan;84(1):144-6. doi: 10.1016/j.mimet.2010.11.001. Epub 2010 Nov 13.

Authors

Mohammad Mehdi Feizabadi¹, Arash Ghodousi, Bizhan Nomanpour, Maryam Omrani, Fereshteh Shahcheraghi

Affiliation

¹ Department for Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. mfeizabadi@tums.ac.ir

PMID: 21078344
DOI: 10.1016/j.mimet.2010.11.001

Abstract

A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Staphylococcus aureus and enterococci to reduce the cost of using lysostaphin. This protocol reduces the expenses of PFGE typing of S. aureus and enterococci as it removes the use of lysostaphin during the spheroplast formation from these bacteria.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques / economics
Bacterial Typing Techniques / methods*
Bacteriological Techniques / economics
Bacteriological Techniques / methods*
DNA, Bacterial / genetics
DNA, Bacterial / isolation & purification*
Electrophoresis, Gel, Pulsed-Field / economics
Electrophoresis, Gel, Pulsed-Field / methods*
Enterococcus / classification
Enterococcus / genetics*
Humans
Lysostaphin / economics
Lysostaphin / metabolism
Staphylococcus aureus / classification
Staphylococcus aureus / genetics*

Substances

DNA, Bacterial
Lysostaphin