The complete genome sequence of a Passion fruit woodiness virus isolate from Australia determined using deep sequencing, and its relationship to other potyviruses

Arch Virol. 2011 Mar;156(3):479-82. doi: 10.1007/s00705-010-0845-3. Epub 2010 Nov 13.

Abstract

The complete genome sequence (9,858 nucleotides) of the Passion fruit woodiness virus isolate MU-2 was determined using Illumina sequencing. The large open reading frame (ORF) encodes a polyprotein containing 3,086 amino acids, with an AUG start codon and UAA stop codon. The polyprotein yielded 11 proteins (P1, HC-Pro, P3, PIPO, 6K1, CI, 6K2, NIa-VPg, NIa-Pro, NIb and CP). Putative cleavage sites between them were identified by sequence comparison to those of other known potyviruses. Accuracy of the genome sequence information was provided by 42-1691-fold sequence coverage, and viral RNA accounted for 7.38% of total polyadenylated RNA from the host plant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Australia
  • Cluster Analysis
  • Genome, Viral*
  • High-Throughput Nucleotide Sequencing*
  • Molecular Sequence Data
  • Phylogeny
  • Polyproteins / genetics
  • Potyvirus / classification*
  • Potyvirus / genetics*
  • Potyvirus / isolation & purification
  • RNA, Viral / genetics*
  • Sequence Analysis, DNA*
  • Viral Proteins / genetics

Substances

  • Polyproteins
  • RNA, Viral
  • Viral Proteins

Associated data

  • GENBANK/HQ122652