Abstract
Oligotrophic denitrifying bacteria, including those belonging to the genera Herbaspirillum, Azospirillum, and Bradyrhizobium, were obtained using a single-cell isolation technique. The taxonomic composition of the denitrifier population was similar to those assessed by previous culture-independent studies. The sequencing of nitrite reductase and N(2)O reductase genes of these strains revealed previously unknown links between 16S rRNA and the denitrification-functional gene phylogenies. In particular, we identified Bradyrhizobium strains that harbor nirS sequences previously detected only in culture-independent studies.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Azospirillum / genetics*
-
Azospirillum / isolation & purification
-
Azospirillum / metabolism
-
Bradyrhizobium / genetics*
-
Bradyrhizobium / isolation & purification
-
Bradyrhizobium / metabolism
-
Cluster Analysis
-
DNA, Bacterial / chemistry
-
DNA, Bacterial / genetics
-
Denitrification*
-
Genes, Bacterial*
-
Herbaspirillum / genetics*
-
Herbaspirillum / isolation & purification
-
Herbaspirillum / metabolism
-
Metabolic Networks and Pathways / genetics*
-
Molecular Sequence Data
-
Nitrite Reductases / genetics
-
Oxidoreductases / genetics
-
Phylogeny
-
RNA, Ribosomal, 16S / genetics
-
Sequence Analysis, DNA
Substances
-
DNA, Bacterial
-
RNA, Ribosomal, 16S
-
Oxidoreductases
-
Nitrite Reductases
-
nitric-oxide reductase