LC/MS data processing for label-free quantitative analysis

Patricia M Palagi; Markus Müller; Daniel Walther; Frédérique Lisacek

doi:10.1007/978-1-60761-987-1_24

LC/MS data processing for label-free quantitative analysis

Methods Mol Biol. 2011:696:369-77. doi: 10.1007/978-1-60761-987-1_24.

Authors

Patricia M Palagi¹, Markus Müller, Daniel Walther, Frédérique Lisacek

Affiliation

¹ Proteome Informatics Group, Swiss Institute of Bioinformatics, Geneva, Switzerland.

PMID: 21063961
DOI: 10.1007/978-1-60761-987-1_24

Abstract

In this chapter, we describe the use of SuperHirn and MSight, two complementary tools developed to the processing of label-free LC/MS data in view of the quantitation of proteomics samples. While MSight is mainly dedicated to the visualisation and navigation into LC/MS data, SuperHirn is specialised in peak detection, normalisation and alignment of LC/MS runs. These two tools can be used in a complementary way and one of the possible usages is described here.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Liquid / methods*
Databases, Protein
Mass Spectrometry / methods*
Proteins / analysis*
Software
Staining and Labeling
Statistics as Topic*

Substances

Proteins