[N-azidomethylbenzoyl blocking group in the phosphotriester synthesis of oligonucleotides]

V A Efimov; A V Aralov; S A Grachev; O G Chakhmakhcheva

doi:10.1134/s1068162010050110

[N-azidomethylbenzoyl blocking group in the phosphotriester synthesis of oligonucleotides]

Bioorg Khim. 2010 Sep-Oct;36(5):681-7. doi: 10.1134/s1068162010050110.

[Article in Russian]

Authors

V A Efimov, A V Aralov, S A Grachev, O G Chakhmakhcheva

PMID: 21063455
DOI: 10.1134/s1068162010050110

Abstract

An effective modification of phosphotriester method for automatic synthesis of DNA and RNA fragments using O-nucleophilic intramolecular catalysis and 2-(azidometil)benzoyl group to protect amino groups of heterocyclic bases of nucleotides is described.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Azides / chemistry*
Catalysis
Electrophoresis, Polyacrylamide Gel
Molecular Structure
Oligonucleotides / chemical synthesis*
Oligonucleotides / chemistry
Organophosphorus Compounds / chemistry*

Substances

Azides
Oligonucleotides
Organophosphorus Compounds