[Identification of allele variants of cattle milk productivity genes using PCR anti-primer method]

Tsitol Genet. 2010 Sep-Oct;44(5):13-7.
[Article in Ukrainian]

Abstract

In this work we have demonstrated two independent real-time PCR methods for detection single nucleotide polymorphism (SNP) of genes csn and acyl-CoA: diacylglycerol acyltransferase 1 (dgat) in cattle. We have analyzed 296 samples of milk production cattle of Ukrainian breeding. The genotype frequencies were AA--0.58, AB--0.34, BB--0.08 for csn gene and for dgat gene--AA--0.7, AK--0.26, KK--0.04. High efficiency of so called "anti-primer" method was shown. Duration of anti-primer PCR reaction was about 2-2.5 hours only and provided full investigation of unknown gene allele.

Publication types

  • English Abstract

MeSH terms

  • Alleles
  • Animals
  • Caseins / genetics*
  • Cattle
  • DNA Primers*
  • Dairying
  • Diacylglycerol O-Acyltransferase / genetics*
  • Female
  • Gene Frequency
  • Lactation / genetics*
  • Polymorphism, Single Nucleotide*
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Ukraine

Substances

  • Caseins
  • DNA Primers
  • Diacylglycerol O-Acyltransferase