Using the Man(9)(GlcNAc)(2)-DC-SIGN pairing to probe specificity in photochemical immobilization

Mol Biosyst. 2011 Jan;7(1):116-8. doi: 10.1039/c0mb00118j. Epub 2010 Nov 9.

Abstract

We demonstrate the expected preference of an immobilised oligosaccharide Man(9)(GlcNAc)(2) upon a 96-well photochemical array, for its known receptor, the cell-surface lectin Dendritic Cell-Specific ICAM3 Grabbing Nonintegrin (DC-SIGN) when compared to immobilised competing monosaccharides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Cell Adhesion Molecules / metabolism*
  • Humans
  • Lectins, C-Type / metabolism*
  • Mannose / chemistry*
  • Molecular Sequence Data
  • Oligosaccharides / chemistry*
  • Oligosaccharides / metabolism*
  • Photochemistry / methods*
  • Receptors, Cell Surface / metabolism*

Substances

  • Cell Adhesion Molecules
  • DC-specific ICAM-3 grabbing nonintegrin
  • Lectins, C-Type
  • Oligosaccharides
  • Receptors, Cell Surface
  • Mannose