Aim: To study the anti-schistosomiasis effect in mouse immunized with rSj26GST vaccine.
Methods: The E.coli BL21/pET28a of rSj26GST gene was coated LB/Kana/IPTG/X-gal pan, cultured, collecting bacteria, bacteria broken, separation, purification. 5 μL purified protein were carried out from SDS-PAGE analysis, then to observe the results of purified protein. Each mouse in infection control group was boosted with 50 μL PBS on 0 weeks later, 2 weeks later and 4 weeks later. While each mice rSj26GST vaccine group was boosted with rSj26GST vaccine. Two weeks after last immunization, each mouse was challenged with 30 ± 2 cercariae of S. japonicum Chinese strain. The control group wasn't do some treatment. The mice were sacrificed at the 45th day post-infection. The size of single egg granulomas in livers was measured with micrometer. The level of hyaluronic acid (HA) and laminin (LN) in sera of the mice was determined by ELISA.
Results: The mean hepatic egg granuloma diameters of the rSj26GST vaccine group(191.6 ± 26.3) μm was significantly smaller than that in infection control group (267.7 ± 28.6) μm (P<0.05). The level of HA and LN in sera of rSj26GST vaccine vaccinated mice was markedly lower than in infection control group (P<0.05).
Conclusion: The results showed that rSj26GST vaccine may act as an effective inhibitor against formation of egg granuloma and reduce immunopathological damage caused by Schistosoma japonicum in the host.