Abstract
This study introduces a simple colorimetric method which can measure the antimicrobial susceptibility of bacteria in biofilms using trimethyl tetrazolium chloride (TTC) as an indicator of viable bacteria. The new method was utilized for the evaluation of antibiotic susceptibility of Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus biofilms.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Infective Agents / pharmacology*
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Biofilms / drug effects*
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Biofilms / growth & development*
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Colorimetry / methods*
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Escherichia coli / drug effects*
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Escherichia coli / physiology
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Formazans / metabolism
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Klebsiella pneumoniae / drug effects*
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Klebsiella pneumoniae / physiology
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Microbial Sensitivity Tests / methods
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Microbial Viability / drug effects
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / physiology
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Tetrazolium Salts / metabolism
Substances
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Anti-Infective Agents
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Formazans
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Tetrazolium Salts