Comparison of muscle fluid and serum for detection of antibodies against hepatitis E virus in slaughter pigs

Vet J. 2011 Oct;190(1):179-80. doi: 10.1016/j.tvjl.2010.09.030. Epub 2010 Nov 1.

Abstract

Muscle fluid was investigated as an alternative to serum for detecting anti-hepatitis E virus (HEV) antibodies in slaughter pigs. Samples of serum and diaphragmatic muscle juice from 67 pigs were analysed by anti-HEV IgG ELISA. Compared to the serum ELISA, the ELISA on diaphragmatic muscle fluid had a sensitivity of 93%, a specificity of 91.7%, a κ value of 0.84 and a determination coefficient (R(2)) of 0.77; both tests had global agreement of results. Muscle fluid can be used as an alternative to serum for serological detection of HEV antibodies in slaughter pigs.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / analysis*
  • Antibodies, Viral / blood
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Hepatitis E / blood
  • Hepatitis E / diagnosis
  • Hepatitis E / immunology
  • Hepatitis E / veterinary*
  • Hepatitis E virus / immunology
  • Hepatitis E virus / isolation & purification*
  • Muscle, Skeletal / immunology*
  • Sensitivity and Specificity
  • Serum / immunology*
  • Spain
  • Sus scrofa
  • Swine
  • Swine Diseases / blood
  • Swine Diseases / diagnosis*
  • Swine Diseases / immunology

Substances

  • Antibodies, Viral