Visualization of color anatomy and molecular fluorescence in whole-mouse cryo-imaging

Comput Med Imaging Graph. 2011 Apr;35(3):195-205. doi: 10.1016/j.compmedimag.2010.10.003. Epub 2010 Oct 30.

Abstract

We developed multi-scale, live-time interactive visualization of color image data, including microscopic whole-mouse cryo-images serving many biomedical applications. Using true-color volume rendering, we interactively, selectively enhanced anatomy using feature detection. For example, to enhance red organs (vessels, liver, etc.) and internal surfaces, we computed a red feature from R/(R+G+B) and surface features from color/gray-scale gradients, respectively. For >70GB cryo-image volumes, we developed multi-resolution visualization, which provided low-resolution rendering of an entire mouse and zooming to organs, tissues, and cells. Fusions of fluorescence and color cryo-volumes uniquely showed biodistribution of metastatic and stem cells within an anatomical context.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Colorimetry / methods*
  • Colorimetry / veterinary*
  • Cryopreservation / veterinary*
  • Image Enhancement / methods
  • Image Interpretation, Computer-Assisted / methods*
  • Mice
  • Microscopy, Fluorescence / methods*
  • Microscopy, Fluorescence / veterinary*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Whole Body Imaging / methods*
  • Whole Body Imaging / veterinary*