A new isolate of Nosema sp. (Microsporidia, Nosematidae) from Phyllobrotica armata Baly (Coleoptera, Chrysomelidae) from China

Feng Zhu; Zhongyuan Shen; Xijie Guo; Xiaofang Xu; Hengping Tao; Xudong Tang; Li Xu

doi:10.1016/j.jip.2010.10.005

A new isolate of Nosema sp. (Microsporidia, Nosematidae) from Phyllobrotica armata Baly (Coleoptera, Chrysomelidae) from China

J Invertebr Pathol. 2011 Feb;106(2):339-42. doi: 10.1016/j.jip.2010.10.005. Epub 2010 Oct 28.

Authors

Feng Zhu¹, Zhongyuan Shen, Xijie Guo, Xiaofang Xu, Hengping Tao, Xudong Tang, Li Xu

Affiliation

¹ Jiangsu University of Science and Technology, Zhenjiang 212018, China.

PMID: 21035452
DOI: 10.1016/j.jip.2010.10.005

Abstract

We studied the spore morphology and molecular systematics of a novel microsporidian isolate from Phyllobrotica armata Baly collected in China. The spores were long-oval and measured 4.7 × 2.6 μm on fresh smears. Ultrastructure of the spores was characteristic for the genus Nosema: 13-14 polar filament coils, posterior vacuole, and a diplokaryon. The complete rRNA gene sequence of the isolate was 4308 bp long. The organization of the rRNA gene was 5'-LSU rRNA-ITS-SSU rRNA-IGS-5S-3', which corresponds to that of the Nosema species. Phylogenetic analysis based on the rRNA gene sequence indicated that this isolate, designated as Nosema sp. PA, is closely related to Nosema bombycis and is correctly assigned to the "true"Nosema group.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
China / epidemiology
Coleoptera / microbiology*
Mycoses / epidemiology
Nosema / genetics
Nosema / isolation & purification*
Phylogeny
RNA, Fungal / genetics
Spores, Fungal / ultrastructure

Substances

RNA, Fungal