Constraction of a subtracted cDNA library using oligo(dT)-latex

E Hara; T Kato; S Nakada; T Nakajima; H Tsurui; K Oda

Constraction of a subtracted cDNA library using oligo(dT)-latex

Nucleic Acids Symp Ser. 1990:(22):29-30.

Authors

E Hara¹, T Kato, S Nakada, T Nakajima, H Tsurui, K Oda

Affiliation

¹ Department of Applied Biological Science, Science University of Tokyo, Chiba, Japan.

PMID: 2101904

Abstract

An efficient method for constraction of subtracted cDNA library was developed using oligo(dT 30. Latex and PCR. This method improved the chances for identifying cDNA clones corresponding to scarce class of mRNA that is expressed differentially during cellular growth and differentiation.

MeSH terms

Acetamides
Base Sequence
Cell Differentiation / genetics
Cloning, Molecular / methods*
DNA
Gene Library*
Humans
Latex
Molecular Sequence Data
Nucleic Acid Hybridization
Oligodeoxyribonucleotides*
Polymerase Chain Reaction
RNA, Messenger / analysis
RNA, Messenger / biosynthesis
Tumor Cells, Cultured

Substances

Acetamides
Latex
Oligodeoxyribonucleotides
RNA, Messenger
oligo (dT)
DNA
hexamethylene bisacetamide