Improved selective and differential medium for isolation of Escherichia coli O157:H7

Sang-Hyun Park; Sangryeol Ryu; Dong-Hyun Kang

doi:10.1128/JCM.01359-10

Improved selective and differential medium for isolation of Escherichia coli O157:H7

J Clin Microbiol. 2011 Jan;49(1):405-8. doi: 10.1128/JCM.01359-10. Epub 2010 Oct 27.

Authors

Sang-Hyun Park¹, Sangryeol Ryu, Dong-Hyun Kang

Affiliation

¹ Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea.

Abstract

GMAC, a modified version of Sorbitol MacConkey medium (SMAC), was produced with a reduced quantity of selective agents and incorporated gentiobiose. GMAC supported a higher recovery rate of heat- or acid-injured Escherichia coli O157:H7 cells than SMAC with cefixime and tellurite (CT-SMAC), while differentiating E. coli O157:H7 from sorbitol-nonfermenting Hafnia alvei.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Culture Media / chemistry*
Escherichia coli Infections / microbiology*
Escherichia coli O157 / classification
Escherichia coli O157 / growth & development
Escherichia coli O157 / isolation & purification*
Hafnia alvei / classification
Hafnia alvei / isolation & purification
Humans
Selection, Genetic

Substances

Culture Media