Abstract
We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Animals
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Basic Helix-Loop-Helix Transcription Factors / genetics
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Carrier Proteins / physiology
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Embryonic Stem Cells / metabolism*
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Genetic Engineering / methods*
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Intracellular Signaling Peptides and Proteins
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Mice
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Nerve Tissue Proteins / physiology
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Nuclear Proteins / physiology
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Promoter Regions, Genetic
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Recombinases / physiology*
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Recombination, Genetic
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Smad4 Protein / genetics
Substances
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Basic Helix-Loop-Helix Transcription Factors
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Carrier Proteins
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Hand2 protein, mouse
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Intracellular Signaling Peptides and Proteins
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Nerve Tissue Proteins
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Nuclear Proteins
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Recombinases
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Smad4 Protein
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Smad4 protein, mouse
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Zfp503 protein, mouse