A method for achieving super-resolved widefield CARS microscopy

Opt Express. 2010 Aug 30;18(18):19263-72. doi: 10.1364/OE.18.019263.

Abstract

We propose a scheme for achieving widefield coherent anti-Stokes Raman scattering (CARS) microscopy images with sub-diffraction-limited resolution. This approach adds structured illumination to the widefield CARS configuration [Applied Physics Letters 84, 816 (2004)]. By capturing a number of images at different phases of the standing wave pattern, an image with up to three times the resolution of the original can be constructed. We develop a theoretical treatment of this system and perform numerical simulations for a typical CARS system, which indicate that resolutions around 120 nm are obtainable with the present scheme. As an imaging system, this method combines the advantages of sub-diffraction-limited resolution, endogenous contrast generation, and a wide field of view.

MeSH terms

  • Algorithms
  • Computer Simulation
  • Diagnostic Imaging
  • Fourier Analysis
  • Image Processing, Computer-Assisted / methods
  • Light
  • Microscopy / methods*
  • Models, Statistical
  • Optics and Photonics*
  • Oscillometry
  • Scattering, Radiation
  • Spectrum Analysis, Raman / methods*