Abstract
The autoimmunity of type 1 diabetes is associated with T-cell hyperactivity. Current study was designed to examine the effect of circulating ribonucleic acids (RNAs), isolated from type 1 diabetic patients on proliferative, apoptotic and inflammatory potential of rat thymocytes. Rat thymocytes were assayed for proliferating nuclear cell antigen (PCNA), Bcl-2, Bax and NF-κB level, using the flow cytometric and fluorometric assays. Cells were allocated into groups, treated with RNAs purified from plasma of juvenile diabetics, adult type 1 diabetic patients, control healthy children, healthy adult persons, nucleic acids and polynucleotide standards (RNA, polyC, PolyA, PolyIC, and CpG). The upregulation of PCNA and Bcl-2 protein and downregulation of Bax protein and NF-κB was shown when the thymocytes where incubated with RNA purified from plasma of juvenile type 1 diabetic patients. The dysregulation of inflammatory cascade and central tolerance may be a defect in autoimmune diseases related to innate immunity leading to corresponding alteration in adaptive immune response.
Copyright © 2010 Elsevier Inc. All rights reserved.
MeSH terms
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Adolescent
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Adult
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Animals
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Cell Proliferation / drug effects
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Cells, Cultured
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Child
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Child, Preschool
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Concanavalin A / pharmacology
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Deoxycytosine Nucleotides / pharmacology
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Deoxyguanosine / analogs & derivatives
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Deoxyguanosine / pharmacology
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Diabetes Mellitus, Type 1 / blood*
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Diabetes Mellitus, Type 1 / genetics
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Humans
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Male
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Middle Aged
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NF-kappa B / metabolism
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Oligonucleotides / blood
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Oligonucleotides / isolation & purification
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Oligonucleotides / pharmacology
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Plasma / chemistry
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Poly I-C / pharmacology
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Polyribonucleotides / pharmacology
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Proliferating Cell Nuclear Antigen / metabolism
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Proto-Oncogene Proteins c-bcl-2 / metabolism
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RNA / blood*
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RNA / isolation & purification
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RNA / pharmacology*
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RNA, Ribosomal / pharmacology
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Rats
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Rats, Wistar
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Thymus Gland / cytology*
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Thymus Gland / drug effects
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Young Adult
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bcl-2-Associated X Protein / metabolism
Substances
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Bax protein, rat
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Deoxycytosine Nucleotides
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NF-kappa B
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Oligonucleotides
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Polyribonucleotides
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Proliferating Cell Nuclear Antigen
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Proto-Oncogene Proteins c-bcl-2
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RNA, Ribosomal
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bcl-2-Associated X Protein
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Concanavalin A
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deoxycytidylyl-(3'-5')-deoxyguanosine
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RNA
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Deoxyguanosine
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Poly I-C