[Prenatal sex identification with biotinylated human Y-specific DNA probe]

Hua Xi Yi Ke Da Xue Xue Bao. 1990 Sep;21(3):277-80.
[Article in Chinese]

Abstract

Using biotinylated human Y-specific DNA probe PHY2.1 and Cos 84, we carried out Southern and dot hybridization with human DNA from artificially aborted chorionic villi, and with DNA of peripheral lymphocytes from normal male and female. The results show that PHY2.1 may be applied in prenatal sex identification both by Southern and by dot hybridization but with Cos 84, only Southern hybridization could be used.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotin
  • Blotting, Southern
  • DNA Probes
  • Female
  • Humans
  • Male
  • Organophosphorus Compounds / chemical synthesis
  • Prenatal Diagnosis*
  • Sex Determination Analysis*
  • Toluene / analogs & derivatives
  • Toluene / chemical synthesis
  • Y Chromosome*

Substances

  • DNA Probes
  • Organophosphorus Compounds
  • Toluene
  • Biotin
  • 4-toluenesulfinic acid