Titanium dioxide (TiO(2)) nanoparticles are widely used in cosmetics, sunscreen and as a photocatalyst. However, little is known about the biological effect of TiO(2) nanoparticles in humans and other animals. Here, we investigated whether prenatal exposure to TiO(2) nanoparticles impacted the central nervous system in mice. We measured the levels of dopamine (DA) and its metabolites in several regions of the brain in mice using high performance liquid chromatography (HPLC). HPLC analysis showed that DA and its metabolites were increased in the prefrontal cortex and the neostriatum following prenatal exposure to TiO(2) nanoparticles. The present study highlights the possibility that maternal exposure to TiO(2) nanoparticles might influence the development of the central dopaminergic system in offspring.