Determination of optical purity of 3,5-dimethoxybenzoyl-leucine diethylamide by chiral chromatography and 1H and 13C NMR spectroscopy

Chirality. 2011 Apr;23(4):281-6. doi: 10.1002/chir.20910. Epub 2010 Oct 6.

Abstract

(R)-N-3,5-dinitrobenzoyl (DNB) leucine derived chiral selector was used as an HPLC chiral stationary phase for the resolution of various racemic amino acids derivatives. In this study, determination of optical purity of an amino acid derivative was performed by chiral high performance liquid chromatography and 1H and 13C NMR spectroscopy by using the DNB leucine derived chiral selector. The accuracy and precision of each optical purity value are calculated and the data are compared to each other.

MeSH terms

  • Amino Acids / chemical synthesis
  • Amino Acids / chemistry*
  • Carbon Isotopes / chemistry
  • Chromatography / methods
  • Chromatography, High Pressure Liquid / methods*
  • Dinitrobenzenes
  • Hydrogen / chemistry*
  • Indicators and Reagents
  • Kinetics
  • Leucine / analogs & derivatives*
  • Leucine / chemistry
  • Magnetic Resonance Spectroscopy / methods*
  • Molecular Structure
  • Sensitivity and Specificity
  • Stereoisomerism

Substances

  • 3,5-dimethoxybenzoyl-leucine diethylamide
  • Amino Acids
  • Carbon Isotopes
  • Dinitrobenzenes
  • Indicators and Reagents
  • dinitrobenzoyl leucine
  • Hydrogen
  • Leucine