Immunological characterization of L-triiodothyronine and L-thyroxine labelled with rhodamine B isothiocyanate

A Reyes; J L Diéguez; M Ruiz; A Alonso; M Morell

Immunological characterization of L-triiodothyronine and L-thyroxine labelled with rhodamine B isothiocyanate

Rev Esp Fisiol. 1990 Sep;46(3):269-72.

Authors

A Reyes¹, J L Diéguez, M Ruiz, A Alonso, M Morell

Affiliation

¹ Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Málaga, Spain.

PMID: 2091151

Abstract

In the present work the label of L-T3 and L-T4 with RBITC have been developed by means of a relatively easy procedure. A purification by TLC, previously optimized, offers the possibility of obtaining a good recovery of conjugates T3 and T4-RBITC. The immunoreactivity of conjugates was calculated by a formulas especially designed for this purpose. This formulas could be applied to any molecule labelled with fluorescent compounds.

Publication types

Comparative Study

MeSH terms

Chromatography, Thin Layer
Fluorescent Dyes*
Radioimmunoassay
Rhodamines*
Spectrometry, Fluorescence
Thyroxine / analysis*
Thyroxine / immunology
Triiodothyronine / analysis*
Triiodothyronine / immunology

Substances

Fluorescent Dyes
Rhodamines
Triiodothyronine
rhodamine isothiocyanate
Thyroxine