Drug-induced permeabilization of S. cerevisiae

Curr Protoc Mol Biol. 2010 Oct:Chapter 13:Unit 13.2B. doi: 10.1002/0471142727.mb1302bs92.

Abstract

Budding yeast are not permeable to many drugs. This unit provides a protocol in which polygodial is used to permeabilize the cell membrane, thereby allowing budding yeast cells to be treated with drugs that otherwise would be ineffective.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents / metabolism*
  • Cell Membrane / drug effects*
  • Cell Membrane / physiology*
  • Cell Membrane Permeability / drug effects*
  • Saccharomyces cerevisiae / drug effects*
  • Saccharomyces cerevisiae / physiology*
  • Sesquiterpenes / metabolism*

Substances

  • Antifungal Agents
  • Sesquiterpenes
  • polygodial