Isothermal detection of RNA with restriction endonucleases

Lei Yan; Shizuka Nakayama; Saron Yitbarek; Isabel Greenfield; Herman O Sintim

doi:10.1039/c0cc02208j

Isothermal detection of RNA with restriction endonucleases

Chem Commun (Camb). 2011 Jan 7;47(1):200-2. doi: 10.1039/c0cc02208j. Epub 2010 Oct 1.

Authors

Lei Yan¹, Shizuka Nakayama, Saron Yitbarek, Isabel Greenfield, Herman O Sintim

Affiliation

¹ Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.

PMID: 20886171
DOI: 10.1039/c0cc02208j

Abstract

Herein, we demonstrate how to detect nucleic acids that do not contain restriction endonuclease recognition sites with restriction endonucleases. We show that the topology of DNA probes used in this detection strategy remarkably affects the efficiency of RNA/DNA detection.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

DNA Probes / chemistry
DNA Restriction Enzymes / chemistry*
DNA Restriction Enzymes / metabolism
RNA / analysis*
Temperature*

Substances

DNA Probes
RNA
DNA Restriction Enzymes