The goal of this study was to investigate the use of Jatropha curcas seed meal (JCSM) in different levels as acaricide in diet of rabbits experimentally infested by Hyalomma marginatum marginatum then determining animal performance, anti-tick feeding and its effects on haemogram of rabbits. Thirty healthy mixed-breed rabbits were randomly divided into five equal groups. The first group was kept as a control fed soya bean meal (20%) as a source of protein. Groups from the second to the fifth fed diets contained 2.5%, 5%, 7.5% and 10% of JCSM instead of soya bean meal as a source of protein, respectively. Feeding and watering were given freely throughout the study. Animal performance for treatment groups were recorded from the 1st week up to the 6th week. Then each group divided into two subgroups, and the ticks were introduced to all of one subgroup and the other kept as control, following them until dropped at the end of the 8th week for all groups of the experiment. Feeding and reproductive performance of the adult tick females were determined. Blood samples were collected and analysed for haematological examination at the 0, 6th and 8th weeks post-treatment from all animals. Result revealed that rabbits received diets containing 5%, 7.5% and 10% had significantly (P < 0.05) lower growth performance than control and 2.5% JCSM groups. Feed intake and body weight gain decreased with increasing the level of JCSM in the diet. The result of anti-tick feeding observed that the highest percentage (60-90%) of rejection was recorded in 10% of JCSM group then the other treated groups (20-30%). Egg mass and reproductive index per female were marked increase (P < 0.001) in groups 2.5%, 5% and 7.5% of JCSM. Macrocytic normochromic anaemia was development after 8 weeks of treatment, which changed to microcytic normochromic anaemia after challenge of ticks in groups received 5%, 7.5% and 10% JCSM. Leukopenia, neutopenia and lymphopenia were noticed (P < 0.05) in all treated groups which more drop in the group received 7.5% JCSM. Also, monocytosis was recorded in 7.5% and 10% JCSM groups. In conclusion, JCSM could be use in the treatment of ectoparasites at level less than 10% in diet. Further investigations should be done to detoxification the Jatropha seed meal to decrease the level of its toxicity.