Concurrent isolation of chikungunya virus and dengue virus from a patient with coinfection resulting from a trip to Singapore

Shu-Fen Chang; Chien-Ling Su; Pei-Yun Shu; Cheng-Fen Yang; Tsai-Ling Liao; Chia-Hsin Cheng; Huai-Chin Hu; Jyh-Hsiung Huang

doi:10.1128/JCM.01228-10

Concurrent isolation of chikungunya virus and dengue virus from a patient with coinfection resulting from a trip to Singapore

J Clin Microbiol. 2010 Dec;48(12):4586-9. doi: 10.1128/JCM.01228-10. Epub 2010 Sep 29.

Authors

Shu-Fen Chang¹, Chien-Ling Su, Pei-Yun Shu, Cheng-Fen Yang, Tsai-Ling Liao, Chia-Hsin Cheng, Huai-Chin Hu, Jyh-Hsiung Huang

Affiliation

¹ Research and Diagnostic Center, Centers for Disease Control, Department of Health, 161, Kun-Yang Street, Taipei, Taiwan, Republic of China.

Abstract

We report two cases of imported infection in patients who had returned to Taiwan from Singapore: one was coinfected with chikungunya virus and dengue virus type 2, and the other was infected with the same dengue virus. Both viruses were successfully isolated from the coinfected case by using antibody neutralization and a plaque purification technique.

Publication types

Case Reports

MeSH terms

Alphavirus Infections / complications*
Alphavirus Infections / diagnosis*
Alphavirus Infections / virology
Chikungunya virus / isolation & purification*
Child
Cluster Analysis
Dengue / complications*
Dengue / diagnosis*
Dengue / virology
Dengue Virus / isolation & purification*
Humans
Male
Molecular Sequence Data
Neutralization Tests
Phylogeny
RNA, Viral / genetics
Sequence Analysis, DNA
Sequence Homology
Singapore
Taiwan
Travel*
Viral Plaque Assay

Substances

RNA, Viral

Associated data

GENBANK/HM067743
GENBANK/HM067746
GENBANK/HM067747