Investigating the structural biofunctionality of antibodies conjugated to magnetic nanoparticles

Nanoscale. 2011 Feb;3(2):387-90. doi: 10.1039/c0nr00436g. Epub 2010 Sep 29.

Abstract

We present the synthesis of trastuzumab-functionalized pegylated iron oxide nanoparticles and provide an FTIR-based approach to gain a direct evidence of the actual conservation of the native structure of conjugated antibody. Their target-selectivity to specific cancer cell receptors has been also assessed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / chemistry*
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal, Humanized
  • Aspartic Acid / analogs & derivatives
  • Aspartic Acid / chemistry
  • Cell Line, Tumor
  • Ferric Compounds / chemistry
  • Fluorescein-5-isothiocyanate / chemistry
  • Humans
  • Immunoconjugates / chemistry
  • Immunoprecipitation
  • Magnetics
  • Metal Nanoparticles / chemistry*
  • Microscopy, Confocal
  • Morpholines / chemistry
  • Spectroscopy, Fourier Transform Infrared
  • Trastuzumab

Substances

  • Antibodies
  • Antibodies, Monoclonal
  • Antibodies, Monoclonal, Humanized
  • Ferric Compounds
  • Immunoconjugates
  • Morpholines
  • poly (3-morpholinopropyl) aspartamide
  • ferric oxide
  • Aspartic Acid
  • Fluorescein-5-isothiocyanate
  • Trastuzumab