Purpose: To observe the effects of the traditional Chinese drug Dipsacus asperoides on the construction of paradentium as well as the mechanism of action to osteoclast during the orthodontic tooth movement in rats.
Methods: Forty-eight female 8-week-old SPF Wistar rats were selected. They were randomly divided into 2 groups: Dipsacus asperoides group and control group. Then animal model for orthodontic tooth movement was established. 3 mL of NS was drenched to the control group and the Dipsacus asperoides group was given 6 g/kg per day Dipsacus asperoides decoction. The rats were executed in batch on the 7th, 14th, 21st, 28th day during the orthodontic treatment. The distances of the tooth movement were measured and slices from the periodontium of the maxillary first molar were observed under optical microscope. Measurement data were compared with t test and analysis of variance by PASW statistics18.
Results: The movement distance of the first molar was significantly larger in the Dipsacus asperoides group than that in the control group. The amount of osteoclast in Dipsacus asperoides group increased significantly compared with that in the control group (P<0.05).
Conclusions: Dipsacus asperoides decoction is useful for the proliferation and differentiation of the osteoclast in orthodontic tooth movement. Supported by Shandong Provincial Science and Technology Development Project (Grant No.2008GG2NS02013) and Natural Science Foundation of Shandong Province (Grant No.Q2008C12).