MhyADH catalysed Michael addition of water and in situ oxidation

Jianfeng Jin; Philip C Oskam; Sanjib K Karmee; Adrie J J Straathof; Ulf Hanefeld

doi:10.1039/c0cc03229h

MhyADH catalysed Michael addition of water and in situ oxidation

Chem Commun (Camb). 2010 Dec 7;46(45):8588-90. doi: 10.1039/c0cc03229h. Epub 2010 Sep 27.

Authors

Jianfeng Jin¹, Philip C Oskam, Sanjib K Karmee, Adrie J J Straathof, Ulf Hanefeld

Affiliation

¹ Gebouw voor Scheikunde, Afdeling Biotechnologie, Technische Universiteit Delft, Julianalaan 136, 2628 BL Delft, The Netherlands.

PMID: 20871891
DOI: 10.1039/c0cc03229h

Abstract

The Michael addition of water is a major challenge. Here an enzymatic approach is described. Interestingly, the enzyme (MhyADH) does not only catalyse the Michael addition of water but also the in situ oxidation of the product.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcohol Dehydrogenase / metabolism*
Betaproteobacteria / enzymology
Biocatalysis*
Oxidation-Reduction
Stereoisomerism
Water / chemistry*
Water / metabolism

Substances

Water
Alcohol Dehydrogenase