[The expression of toll-like receptor4 and NF-kappaB in ulcerative colitis]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Jul;26(7):650-2.
[Article in Chinese]

Abstract

Aim: To investigate the expression of TLR4 and NF-kappaB in ulcerative colitis (UC).

Methods: Colonic biopsy specimens were collected from active UC and controls. The expression of TLR4 and NF-kappaBp 65 were analyzed by immunohistochemistry (IHC) and RT-PCR.

Results: RT-PCR revealed a significant increase of TLR4 and NF-kappaBp 65 antigen expression in colonic mucosa of UC compared with colonic mucosa of controls (TLR4: 143.658+/-33.870, 30.531+/-8.442, t=24.253, P<0.01; NF-kappaBp65: 185.773+/-37.625, 23.810+/-7.038, t=31.664, P<0.01). IHC show that the expression of TLR4 and NF-kappaB was significantly higher in colonic mucosa of UC compared with colonic mucosa of controls.

Conclusion: The expression of the TLR4 and NF-kappaB was increased in the colonic mucosa of UC compared with colonic mucosa of controls. It may be closely associated with the course of ulcerative colitis.

Publication types

  • English Abstract

MeSH terms

  • Colitis, Ulcerative / genetics*
  • Colitis, Ulcerative / metabolism
  • Gene Expression*
  • Humans
  • Intestinal Mucosa / metabolism
  • Toll-Like Receptor 4 / genetics*
  • Toll-Like Receptor 4 / metabolism
  • Transcription Factor RelA / genetics*
  • Transcription Factor RelA / metabolism

Substances

  • TLR4 protein, human
  • Toll-Like Receptor 4
  • Transcription Factor RelA