Nucleotide regulation of vasoactive intestinal peptide binding to bovine thyroid plasma membranes

J R Calvo; M F Morales; J C Mesa; R Goberna

doi:10.1007/BF01116612

Nucleotide regulation of vasoactive intestinal peptide binding to bovine thyroid plasma membranes

Biosci Rep. 1990 Dec;10(6):519-25. doi: 10.1007/BF01116612.

Authors

J R Calvo¹, M F Morales, J C Mesa, R Goberna

Affiliation

¹ Department of Medical Biochemistry and Molecular Biology, School of Medicine, University of Sevilla, Spain.

PMID: 2085668
DOI: 10.1007/BF01116612

Abstract

The specific binding of vasoactive intestinal peptide (VIP) to bovine thyroid plasma membranes is inhibited by guanine nucleotides. Guanosine 5'-triphosphate (GTP) and the non-hydrolyzable GTP analogs guanosine 5'-beta,gamma-imidotriphosphate (Gpp(NH)p) and guanosine 5'-O-(3-thiotriphosphate) (GTP-gamma-S) inhibited markedly the binding of VIP to its receptors. This inhibition was higher with GTP than with Gpp(NH)p and GTP-gamma-S and was due to an increase of the rate of dissociation of peptide bound to membranes. Other nucleotides did not show any effect.

MeSH terms

Animals
Binding Sites / drug effects
Cattle
Cell Membrane / metabolism
Dose-Response Relationship, Drug
Guanosine Triphosphate / analogs & derivatives
Guanosine Triphosphate / pharmacology*
Thyroid Gland / drug effects
Thyroid Gland / metabolism*
Vasoactive Intestinal Peptide / metabolism*

Substances

Vasoactive Intestinal Peptide
Guanosine Triphosphate