Abstract
Solution-phase spectroscopy and mass spectrometry are used to probe interactions between divalent metal ions and a synthetic Cys(2)His(2) zinc-finger peptide (vCP1). Both methods provide the same order of binding affinity, zinc ≥ cobalt ≫ copper ≫ calcium. Collision-cross-section measurements show that both apo and holo forms are compact. This is corroborated by molecular-dynamics simulations.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Calcium / chemistry*
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Cobalt / chemistry*
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Copper / chemistry*
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Cysteine / chemistry
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Gases / chemistry
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Histidine / chemistry
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Models, Molecular
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Molecular Dynamics Simulation
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Peptides / chemical synthesis
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Peptides / chemistry*
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Solutions
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Zinc / chemistry*
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Zinc Fingers*
Substances
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Gases
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Peptides
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Solutions
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Cobalt
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Histidine
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Copper
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Zinc
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Cysteine
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Calcium