Metal binding to a zinc-finger peptide: a comparison between solution and the gas phase

Chem Commun (Camb). 2011 Jan 7;47(1):412-4. doi: 10.1039/c0cc02445g. Epub 2010 Sep 20.

Abstract

Solution-phase spectroscopy and mass spectrometry are used to probe interactions between divalent metal ions and a synthetic Cys(2)His(2) zinc-finger peptide (vCP1). Both methods provide the same order of binding affinity, zinc ≥ cobalt ≫ copper ≫ calcium. Collision-cross-section measurements show that both apo and holo forms are compact. This is corroborated by molecular-dynamics simulations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium / chemistry*
  • Cobalt / chemistry*
  • Copper / chemistry*
  • Cysteine / chemistry
  • Gases / chemistry
  • Histidine / chemistry
  • Models, Molecular
  • Molecular Dynamics Simulation
  • Peptides / chemical synthesis
  • Peptides / chemistry*
  • Solutions
  • Zinc / chemistry*
  • Zinc Fingers*

Substances

  • Gases
  • Peptides
  • Solutions
  • Cobalt
  • Histidine
  • Copper
  • Zinc
  • Cysteine
  • Calcium