Abstract
A real-time quantitative PCR method was developed for the detection and enumeration of Mycobacterium spp. from environmental samples and was compared to two other methods already described. The results showed that our method, targeting 16S rRNA, was more specific than the two previously published real-time quantitative PCR methods targeting another 16S rRNA locus and the hsp65 gene (100% versus 44% and 91%, respectively).
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Load / methods
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DNA, Bacterial / genetics
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Genes, Bacterial / genetics
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Molecular Sequence Data
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Mycobacterium / genetics*
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Polymerase Chain Reaction / methods*
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RNA, Ribosomal, 16S / genetics
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Sensitivity and Specificity
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Water Microbiology*
Substances
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DNA, Bacterial
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RNA, Ribosomal, 16S
Associated data
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