Functionality of sortase A in Lactococcus lactis

Yakhya Dieye; Virginie Oxaran; Florence Ledue-Clier; Walid Alkhalaf; Girbe Buist; Vincent Juillard; Chang Won Lee; Jean-Christophe Piard

doi:10.1128/AEM.00928-10

Functionality of sortase A in Lactococcus lactis

Appl Environ Microbiol. 2010 Nov;76(21):7332-7. doi: 10.1128/AEM.00928-10. Epub 2010 Sep 17.

Authors

Yakhya Dieye¹, Virginie Oxaran, Florence Ledue-Clier, Walid Alkhalaf, Girbe Buist, Vincent Juillard, Chang Won Lee, Jean-Christophe Piard

Affiliation

¹ INRA, UMR1319 Micalis, Domaine de Vilvert, F-78352 Jouy-en-Josas, France.

Abstract

Lactococcus lactis IL1403 harbors a putative sortase A (SrtA) and 11 putative sortase substrates that carry the canonical LPXTG signature of such substrates. We report here on the functionality of SrtA to anchor five LPXTG substrates to the cell wall, thus suggesting that SrtA is the housekeeping sortase in L. lactis IL1403.

MeSH terms

Aminoacyltransferases / metabolism
Aminoacyltransferases / physiology*
Bacterial Adhesion / genetics
Bacterial Adhesion / physiology
Bacterial Proteins / metabolism
Bacterial Proteins / physiology*
Cell Wall / metabolism
Cell Wall / physiology
Cysteine Endopeptidases / metabolism
Cysteine Endopeptidases / physiology*
Genes, Bacterial / genetics
Lactococcus lactis / enzymology*
Lactococcus lactis / genetics
Lactococcus lactis / metabolism
Lactococcus lactis / physiology
Mutagenesis, Site-Directed
Protein Transport / genetics
Proteome / genetics
Recombinant Fusion Proteins / genetics

Substances

Bacterial Proteins
Proteome
Recombinant Fusion Proteins
Aminoacyltransferases
sortase A
Cysteine Endopeptidases