Abstract
Two immunocompromised patients with 2009 H1N1 influenza pneumonia had viral shedding for over 5 weeks despite therapy with oseltamivir. Declining or persistently low cycle threshold values noted on serial qualitative real-time reverse transcriptase PCR (rRT-PCR) of respiratory specimens implied increasing viral load and probable drug resistance. Oseltamivir resistance was later confirmed by pyrosequencing.
MeSH terms
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Antiviral Agents / administration & dosage
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Antiviral Agents / pharmacology*
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Drug Resistance, Viral*
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Humans
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Immunocompromised Host
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Influenza A Virus, H1N1 Subtype / drug effects*
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Influenza A Virus, H1N1 Subtype / genetics
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Influenza A Virus, H1N1 Subtype / isolation & purification
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Influenza, Human / drug therapy*
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Influenza, Human / virology
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Male
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Microbial Sensitivity Tests
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Middle Aged
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Oseltamivir / administration & dosage
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Oseltamivir / pharmacology*
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Sequence Analysis, DNA
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Sputum / virology
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Time Factors
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Viral Load
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Virus Shedding
Substances
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Antiviral Agents
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Oseltamivir