Overview of quantitative LC-MS techniques for proteomics and activitomics

John F Timms; Pedro R Cutillas

doi:10.1007/978-1-60761-780-8_2

Overview of quantitative LC-MS techniques for proteomics and activitomics

Methods Mol Biol. 2010:658:19-45. doi: 10.1007/978-1-60761-780-8_2.

Authors

John F Timms¹, Pedro R Cutillas

Affiliation

¹ Cancer Proteomics Laboratory, EGA Institute for Women's Health, University College London, London, UK.

PMID: 20839096
DOI: 10.1007/978-1-60761-780-8_2

Abstract

LC-MS is a useful technique for protein and peptide quantification. In addition, as a powerful tool for systems biology research, LC-MS can also be used to quantify post-translational modifications and metabolites that reflect biochemical pathway activity. This review discusses the different analytical techniques that use LC-MS for the quantification of proteins, their modifications and activities in a multiplex manner.

Publication types

Review

MeSH terms

Animals
Chromatography, Liquid / methods*
Humans
Mass Spectrometry / methods*
Protein Processing, Post-Translational
Proteins / analysis
Proteins / chemistry
Proteins / metabolism*
Proteomics / methods*
Staining and Labeling

Substances

Proteins