Abstract
We report a general method based on wide-field fluorescence imaging of single molecule photobleaching and the Chung-Kennedy algorithm to measure the stoichiometry of functional protein complexes in living bacterial cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Algorithms
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Bacterial Proteins / chemistry*
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Bacterial Proteins / metabolism
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Escherichia coli / chemistry*
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Escherichia coli / cytology
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Escherichia coli / metabolism
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Fluorescent Dyes / chemistry*
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Heat-Shock Proteins / chemistry*
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Heat-Shock Proteins / metabolism
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Image Enhancement
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Microscopy, Fluorescence
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Photobleaching*
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Stereoisomerism
Substances
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Bacterial Proteins
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Fluorescent Dyes
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Heat-Shock Proteins
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phage shock protein, Bacteria