Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching

Chem Commun (Camb). 2011 Jan 7;47(1):400-2. doi: 10.1039/c0cc01707h. Epub 2010 Sep 7.

Abstract

We report a general method based on wide-field fluorescence imaging of single molecule photobleaching and the Chung-Kennedy algorithm to measure the stoichiometry of functional protein complexes in living bacterial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / metabolism
  • Escherichia coli / chemistry*
  • Escherichia coli / cytology
  • Escherichia coli / metabolism
  • Fluorescent Dyes / chemistry*
  • Heat-Shock Proteins / chemistry*
  • Heat-Shock Proteins / metabolism
  • Image Enhancement
  • Microscopy, Fluorescence
  • Photobleaching*
  • Stereoisomerism

Substances

  • Bacterial Proteins
  • Fluorescent Dyes
  • Heat-Shock Proteins
  • phage shock protein, Bacteria