Multicenter evaluation of the BD Max GBS assay for detection of group B streptococci in prenatal vaginal and rectal screening swab specimens from pregnant women

Jennifer Riedlinger; Safedin H Beqaj; Marsha A Milish; Stephen Young; Rebecca Smith; Monique Dodd; Rosemary E Hankerd; William D Lebar; Duane W Newton

doi:10.1128/JCM.00947-10

Multicenter evaluation of the BD Max GBS assay for detection of group B streptococci in prenatal vaginal and rectal screening swab specimens from pregnant women

J Clin Microbiol. 2010 Nov;48(11):4239-41. doi: 10.1128/JCM.00947-10. Epub 2010 Sep 8.

Authors

Jennifer Riedlinger¹, Safedin H Beqaj, Marsha A Milish, Stephen Young, Rebecca Smith, Monique Dodd, Rosemary E Hankerd, William D Lebar, Duane W Newton

Affiliation

¹ BD-HandyLab, Ann Arbor, Michigan, USA.

Abstract

A new integrated extraction and real-time PCR-based system for the detection of group B streptococci in antepartum screening samples enriched in Lim broth was compared to the CDC-recommended culture method. The BD Max GBS assay exhibited acceptable sensitivity (95%) and specificity (96.7%) compared to those of the culture method in this multisite evaluation.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Bacteriological Techniques / methods*
Carrier State / diagnosis*
Carrier State / microbiology
Female
Humans
Mass Screening / methods*
Polymerase Chain Reaction / methods
Pregnancy
Pregnancy Complications, Infectious / diagnosis*
Pregnancy Complications, Infectious / microbiology
Prenatal Care / methods
Reagent Kits, Diagnostic
Rectum / microbiology
Sensitivity and Specificity
Streptococcal Infections / diagnosis*
Streptococcal Infections / microbiology
Streptococcus agalactiae / isolation & purification*
Vagina / microbiology

Substances

Reagent Kits, Diagnostic