Abstract
To determine the anti-inflammatory and analgesic activities of methyl gallate (MG) isolated from Galla Rhois, MG was studied in vivo for its analgesic activities using the writhing response in mice. Anti-inflammatory activity of MG was evaluated for NO and IL-6 production in RAW 264.7 cells. MG inhibited LPS-induced NO and IL-6 production. Consistent with these observations, the protein and mRNA expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were inhibited by MG. Moreover, MG suppressed the phosphorylation of ERK1/2 in LPS-induced RAW 264.7 cells in a dose-dependent manner. Taken together, the results of this study indicate that MG has anti-inflammatory effects.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Analgesics / pharmacology*
-
Animals
-
Anti-Inflammatory Agents / pharmacology*
-
Behavior, Animal / drug effects
-
Cyclooxygenase 2 / genetics
-
Cyclooxygenase 2 / metabolism
-
Dose-Response Relationship, Drug
-
Down-Regulation
-
Drugs, Chinese Herbal / chemistry
-
Drugs, Chinese Herbal / pharmacology*
-
Extracellular Signal-Regulated MAP Kinases / metabolism
-
Gallic Acid / analogs & derivatives*
-
Gallic Acid / isolation & purification
-
Gallic Acid / pharmacology
-
Gallic Acid / therapeutic use
-
Interleukin-6 / biosynthesis*
-
Lipopolysaccharides
-
Male
-
Mice
-
Mice, Inbred ICR
-
Nitric Oxide / biosynthesis*
-
Nitric Oxide Synthase Type II / genetics
-
Nitric Oxide Synthase Type II / metabolism
-
Pain / drug therapy*
-
Phosphorylation
-
Plant Tumors
-
RNA, Messenger / metabolism
-
Signal Transduction / drug effects
Substances
-
Analgesics
-
Anti-Inflammatory Agents
-
Drugs, Chinese Herbal
-
Interleukin-6
-
Lipopolysaccharides
-
RNA, Messenger
-
Nitric Oxide
-
methyl gallate
-
Gallic Acid
-
Nitric Oxide Synthase Type II
-
Cyclooxygenase 2
-
Extracellular Signal-Regulated MAP Kinases