Abstract
Several transgenic tobacco lines expressing human apolipoprotein A-I (ApoA-I) were obtained. Western blot analyses indicated the expression of the recombinant protein in plant organs at various stages of development, including senescent leaves. A cell line expressing human ApoA-I was established from a T(1) transgenic plant. Recombinant ApoA-I was isolated either from extracts of transgenic leaves and from the culture medium of transgenic cells using an antibody-based one-step procedure.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apolipoprotein A-I / biosynthesis*
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Apolipoprotein A-I / genetics
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Apolipoprotein A-I / isolation & purification
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Blotting, Western
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Cell Culture Techniques
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Chromatography, Affinity
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Gene Expression
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Humans
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Nicotiana / genetics
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Nicotiana / metabolism*
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Plant Leaves / genetics
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Plant Leaves / metabolism
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Plants, Genetically Modified / genetics
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Plants, Genetically Modified / metabolism
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
Substances
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APOA1 protein, human
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Apolipoprotein A-I
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Recombinant Proteins