This paper presents a pulse method for determination of resonance frequency of magnetoelastic sensors. The method eliminates the bias field that is necessary in previous methods and also allows fast and accurate detection. The stability tests of the system show an average standard deviation of 129 Hz and an average drift of -10.4 Hz/h. This system allows simultaneous detection of two sensors. A simulation of the operation of one and two sensors was shown to be very similar to the real data plots from the test system. Real tests have shown that adding a second sensor does not affect the resonance frequency of the first sensor. The effect of pulse magnetic field on the characteristics of the resonance signal, including resonance frequency, amplitude, and Q-value of frequency domain signal, has been studied and real time detection using magnetoelastic sensors was demonstrated in a flowing system.